| Pathological anatomy
(objectives)
Are essential objectives: the acquisition of basic knowledge about the main safety standards of the histopathology laboratory, the acquisition of knowledge of the preparation of histological samples for optical and electronic microscopy.
These objectives will be achieved through lectures and interactive teaching activities, designed to facilitate learning and improve the ability to solve problems related to the execution of histocytopathological preparation techniques.
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|
Code
|
90333 |
|
Language
|
ITA |
|
Type of certificate
|
Profit certificate
|
| Module: Fundamentals of histopathology and special histopathology
(objectives)
Essential objectives include the acquisition of basic knowledge about the main safety standards
of the histopathology laboratory, knowledge of histological techniques from sample
fixation/preservation to paraffin embedding, and cytological techniques from sample
fixation/preservation to preparation; basic histochemical and immunohistochemical techniques;
issues related to the preparation of histological and cytological samples and ancillary techniques
(histochemical and immunohistochemical); principles of optics, optical microscopy, and electron
microscopy. These objectives will be achieved through lectures and interactive teaching
activities, designed to facilitate learning and improve the ability to solve problems related to the
execution of histo-cytopathological preparation techniques.
|
|
Language
|
ITA |
|
Type of certificate
|
Profit certificate
|
|
Credits
|
4
|
|
Scientific Disciplinary Sector Code
|
MED/08
|
|
Contact Hours
|
40
|
|
Type of Activity
|
Core compulsory activities
|
|
Teacher
|
Scimeca Manuel
(syllabus)
BASIC HISTOLOGICAL AND CYTOLOGICAL TECHNIQUES:
• Main techniques for histological sample fixation and preparation
• Main techniques for biological fluid sample fixation and preparation
• Techniques for paraffin embedding of histological samples
• Techniques for paraffin embedding and sectioning
• Techniques for fresh sample preparation
• Artifacts from technical preparation
• Principles of operation and use of equipment
• Safety in the Anatomical Pathology Laboratory
ANCILLARY HISTOLOGICAL AND CYTOLOGICAL TECHNIQUES:
• Histochemical staining techniques
• Immunohistochemical investigation techniques
• Immunofluorescence techniques (direct and indirect)
• Introduction to electron microscopy techniques
CELLULAR RESPONSE TO DAMAGE:
• Cellular response to ischemia (definition of ischemia and hypoxia, types of hypoxia:
hypoxic, anemic, stagnant, ischemic, histotoxic)
• Warm and cold ischemia
• Ischemia times and cellular susceptibility
• Ischemic cell damage, hydropic degeneration, vacuolar degeneration, cloudy swelling
• Reversible and irreversible subcellular modifications associated with hydropic swelling
• Microscopic aspects of cell death, concepts of oncosis, coagulative necrosis, liquefactive
necrosis, and apoptosis
PRINCIPLES OF OPTICS:
• Principles of optics
• Converging and diverging lenses
• Chromatic aberrations
• Image formation
MICROSCOPY:
• Principles of optical microscopy
• Brightfield microscopy
• Darkfield microscopy
• Fluorescence microscopy
• Digitalization
ELECTRON MICROSCOPY:
• Principles of electron microscopy
• Applications of ultrastructural microscopy
APPLICATIONS FOR ANATOMICAL-PATHOLOGICAL MOLECULAR DIAGNOSTICS
• FISH and SISH
(reference books)
• Scientific articles and teaching materials selected and provided by the teacher
• Free downloadable book: Practical Histopathology 1st Edition by Shafie Abulkadir Hassan
Title: Laboratory Methods in Histotechnology
Authors: Edna B. Prophet, Armed Forces Institute of Pathology (U.S.)
Editor: Edna B. Prophet
Edition: Reprint
Publisher: American Registry of Pathology, 1992
Source: University of Michigan
Digitized: July 29, 2008
Title: Advanced Laboratory Methods in Histology and Pathology
Author: Ulrika V. Mikel
Editor: Ulrika V. Mikel
Contributor: Armed Forces Institute of Pathology (U.S.)
Illustrated Edition
Publisher: Armed Forces Institute of Pathology, American Registry of Pathology, 1994
Source: University of Michigan
Digitized: July 31, 2008
|
|
Dates of beginning and end of teaching activities
|
From to |
|
Attendance
|
not mandatory
|
|
Evaluation methods
|
Written test
|
|
Teacher
|
Carlino Angela
(syllabus)
BASIC HISTOLOGICAL AND CYTOLOGICAL TECHNIQUES:
• Main techniques for histological sample fixation and preparation
• Main techniques for biological fluid sample fixation and preparation
• Techniques for paraffin embedding of histological samples
• Techniques for paraffin embedding and sectioning
• Techniques for fresh sample preparation
• Artifacts from technical preparation
• Principles of operation and use of equipment
• Safety in the Anatomical Pathology Laboratory
ANCILLARY HISTOLOGICAL AND CYTOLOGICAL TECHNIQUES:
• Histochemical staining techniques
• Immunohistochemical investigation techniques
• Immunofluorescence techniques (direct and indirect)
• Introduction to electron microscopy techniques
CELLULAR RESPONSE TO DAMAGE:
• Cellular response to ischemia (definition of ischemia and hypoxia, types of hypoxia:
hypoxic, anemic, stagnant, ischemic, histotoxic)
• Warm and cold ischemia
• Ischemia times and cellular susceptibility
• Ischemic cell damage, hydropic degeneration, vacuolar degeneration, cloudy swelling
• Reversible and irreversible subcellular modifications associated with hydropic swelling
• Microscopic aspects of cell death, concepts of oncosis, coagulative necrosis, liquefactive
necrosis, and apoptosis
PRINCIPLES OF OPTICS:
• Principles of optics
• Converging and diverging lenses
• Chromatic aberrations
• Image formation
MICROSCOPY:
• Principles of optical microscopy
• Brightfield microscopy
• Darkfield microscopy
• Fluorescence microscopy
• Digitalization
ELECTRON MICROSCOPY:
• Principles of electron microscopy
• Applications of ultrastructural microscopy
APPLICATIONS FOR ANATOMICAL-PATHOLOGICAL MOLECULAR DIAGNOSTICS
• FISH and SISH
(reference books)
• Scientific articles and teaching materials selected and provided by the teacher
• Free downloadable book: Practical Histopathology 1st Edition by Shafie Abulkadir Hassan
Title: Laboratory Methods in Histotechnology
Authors: Edna B. Prophet, Armed Forces Institute of Pathology (U.S.)
Editor: Edna B. Prophet
Edition: Reprint
Publisher: American Registry of Pathology, 1992
Source: University of Michigan
Digitized: July 29, 2008
Title: Advanced Laboratory Methods in Histology and Pathology
Author: Ulrika V. Mikel
Editor: Ulrika V. Mikel
Contributor: Armed Forces Institute of Pathology (U.S.)
Illustrated Edition
Publisher: Armed Forces Institute of Pathology, American Registry of Pathology, 1994
Source: University of Michigan
Digitized: July 31, 2008
|
|
Dates of beginning and end of teaching activities
|
From to |
|
Delivery mode
|
Traditional
|
|
Attendance
|
not mandatory
|
|
Evaluation methods
|
Oral exam
|
|
|
| Module: Technical sciences of medical laboratory-histo-cytopathology
(objectives)
Are essential objectives: the acquisition of basic knowledge about the main safety standards of the histopathology laboratory, the acquisition of knowledge of the preparation of histological samples for optical and electronic microscopy.
These objectives will be achieved through lectures and interactive teaching activities, designed to facilitate learning and improve the ability to solve problems related to the execution of histocytopathological preparation techniques.
|
|
Language
|
ITA |
|
Type of certificate
|
Profit certificate
|
|
Credits
|
1
|
|
Scientific Disciplinary Sector Code
|
MED/46
|
|
Contact Hours
|
10
|
|
Type of Activity
|
Core compulsory activities
|
|
Teacher
|
Virgili Roberto
(syllabus)
BASIC HISTOLOGICAL AND CYTOLOGICAL TECHNIQUES:
• Main techniques for histological sample fixation and preparation
• Main techniques for biological fluid sample fixation and preparation
• Techniques for paraffin embedding of histological samples
• Techniques for paraffin embedding and sectioning
• Techniques for fresh sample preparation
• Artifacts from technical preparation
• Principles of operation and use of equipment
• Safety in the Anatomical Pathology Laboratory
ANCILLARY HISTOLOGICAL AND CYTOLOGICAL TECHNIQUES:
• Histochemical staining techniques
• Immunohistochemical investigation techniques
• Immunofluorescence techniques (direct and indirect)
• Introduction to electron microscopy techniques
CELLULAR RESPONSE TO DAMAGE:
• Cellular response to ischemia (definition of ischemia and hypoxia, types of hypoxia:
hypoxic, anemic, stagnant, ischemic, histotoxic)
• Warm and cold ischemia
• Ischemia times and cellular susceptibility
• Ischemic cell damage, hydropic degeneration, vacuolar degeneration, cloudy swelling
• Reversible and irreversible subcellular modifications associated with hydropic swelling
• Microscopic aspects of cell death, concepts of oncosis, coagulative necrosis, liquefactive
necrosis, and apoptosis
PRINCIPLES OF OPTICS:
• Principles of optics
• Converging and diverging lenses
• Chromatic aberrations
• Image formation
MICROSCOPY:
• Principles of optical microscopy
• Brightfield microscopy
• Darkfield microscopy
• Fluorescence microscopy
• Digitalization
ELECTRON MICROSCOPY:
• Principles of electron microscopy
• Applications of ultrastructural microscopy
APPLICATIONS FOR ANATOMICAL-PATHOLOGICAL MOLECULAR DIAGNOSTICS
• FISH and SISH
(reference books)
• Scientific articles and teaching materials selected and provided by the teacher
• Free downloadable book: Practical Histopathology 1st Edition by Shafie Abulkadir Hassan
Title: Laboratory Methods in Histotechnology
Authors: Edna B. Prophet, Armed Forces Institute of Pathology (U.S.)
Editor: Edna B. Prophet
Edition: Reprint
Publisher: American Registry of Pathology, 1992
Source: University of Michigan
Digitized: July 29, 2008
Title: Advanced Laboratory Methods in Histology and Pathology
Author: Ulrika V. Mikel
Editor: Ulrika V. Mikel
Contributor: Armed Forces Institute of Pathology (U.S.)
Illustrated Edition
Publisher: Armed Forces Institute of Pathology, American Registry of Pathology, 1994
Source: University of Michigan
Digitized: July 31, 2008
|
|
Dates of beginning and end of teaching activities
|
From to |
|
Delivery mode
|
Traditional
|
|
Attendance
|
not mandatory
|
|
Evaluation methods
|
Oral exam
|
|
|
|
