| Microbiological diagnostics
(objectives)
The main educational objective of this course is to provide the student with the basic knowledge of microbiological diagnosis, referring to basic and innovative techniques.This allows the student to acquire an overview of the diagnosis by understanding in which biological matrices the microorganisms must be researched and what precautions to take in order to make accurate laboratory diagnoses in the microbiological field
The student must have acquired skills and learning methods suitable for updating and continually raising his skills both by independently drawing on scientific texts and articles, and by integrating them with the contents of the other teachings and must be able to gradually acquire the ability to attend specialized seminars, conferences, masters, etc.
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Code
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90339 |
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Language
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ITA |
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Type of certificate
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Profit certificate
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| Module: Bacteriological, virological, mycological diagnostics
(objectives)
The main training objective of this teaching is to provide the student with the basic knowledge of
microbiological diagnosis, referring to basic and innovative techniques, aimed at achieving the
training objectives of the professional profile. This enables the student to acquire an overview of
diagnosis by understanding in which biological matrices microorganisms must be sought and whatprecautions must be taken in order to make accurate laboratory diagnoses in the microbiological
field
The main pathogens involved in human pathogenesis will be covered and which laboratory
techniques to adopt depending on the pathogen. Diagnostic algorithms will be illustrated so that
the student will be able to understand the steps of microbiological diagnosis.
By the end of the course, the student will have acquired the main methods and techniques to be
applied for the identification of bacteria, viruses, fungi, parasites and protozoa, with the ability to
apply the most appropriate diagnostic technique.
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Language
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ITA |
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Type of certificate
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Profit certificate
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Credits
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3
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Scientific Disciplinary Sector Code
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MED/07
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Contact Hours
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30
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Type of Activity
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Core compulsory activities
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Teacher
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Di Caro Antonino
(syllabus)
Bacteria, viruses and fungi of medical interest.
-Microbiological diagnostics: pathological samples, collection, transport, storage and processing of samples.
-Bacterial infections, direct microscopic examination and culture.
-Viral infections, direct examination and culture.
-Methods of detection of microbial macromolecules. Latex agglutination test. Elisa. Immunofluorescence.
Immunohistochemistry.
-Serological diagnosis of infection: methods for documenting the presence and titre of specific antibodies.
-Molecular microbiological diagnostics: extraction of nucleic acids, hybridization methods, amplification of
the hybridization signal, post-amplification detection.
-Automation in the clinical microbiology laboratory: automation in serology, identification of bacteria and
antibiogram, extraction of nucleic acids and in amplification of nucleic acids.
-Respiratory tract infections: the main clinical manifestations and the laboratory diagnosis of upper and
lower respiratory tract infections.
-The most common central nervous system infections (meningitis, encephalitis) and their laboratory
diagnosis.
-Infections of the genitourinary system, in relation to the microbes ( bacteria, viruses and fungi) involved
and to the various types of laboratory diagnosis. Sexually transmitted diseases (STDs) and urinary tract
infections (UTIs).
-Gastrointestinal tract infections: the main infections of the stomach, intestines and liver in relation to their
etiologic agents an laboratory diagnosis.
-Skin, bone and joint infections: laboratory diagnostic methods.
-Vascular and cardiac infections, bacteremia, sepsis: main infectious agents and laboratory diagnosis.
-Opportunistic infections and healthcare-related infections (ICA): definitions, risk factors, routes of
transmission, prevention and treatment.
(reference books)
“Lippincott® Illustrated Reviews: Microbiology (Lippincott Illustrated Reviews Series) Cynthia Nau Cornelissen
Ph.D. (Editor), Marcia Metzgar Hobbs PhD (Editor). Series Editor: Harvey RH, Walters Kluwer”
-“Bibliografia e materiale didattico scelti dal docente (presentazioni, articoli scientifici, dispense)”
-“Microbiologia medica, Patrick R. Murray, Ken S. Rosenthal, Michael A. Pfaller, Editore EDRA”
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Dates of beginning and end of teaching activities
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From to |
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Delivery mode
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Traditional
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Attendance
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not mandatory
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Evaluation methods
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Oral exam
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| Module: Parasitological diagnostics
(objectives)
The main training objective of this teaching is to provide the student with the basic knowledge of
microbiological diagnosis, referring to basic and innovative techniques, aimed at achieving the
training objectives of the professional profile. This enables the student to acquire an overview of
diagnosis by understanding in which biological matrices microorganisms must be sought and whatprecautions must be taken in order to make accurate laboratory diagnoses in the microbiological
field
The main pathogens involved in human pathogenesis will be covered and which laboratory
techniques to adopt depending on the pathogen. Diagnostic algorithms will be illustrated so that
the student will be able to understand the steps of microbiological diagnosis.
By the end of the course, the student will have acquired the main methods and techniques to be
applied for the identification of bacteria, viruses, fungi, parasites and protozoa, with the ability to
apply the most appropriate diagnostic technique.
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|
Language
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ITA |
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Type of certificate
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Profit certificate
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Credits
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1
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Scientific Disciplinary Sector Code
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VET/06
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Contact Hours
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10
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Type of Activity
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Core compulsory activities
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Teacher
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Pichler Verena
(syllabus)
- Introduction to the course: General parasitology and ecology concepts applied to parasites and
zoonoses
- Enteric parasites: main parasites (Protozoa: Giardia intestinalis, Amoeba-Entamoeba histolytica,
Sporozoa- Cryptosporidium sp. Cestodes: Taenia solium, Taenia saginata, Diphillobothrium latum,
Digenei hepato-biliary Trematodes- Opisthorchis felineus, Clonorchis sinensis, Nematodes- Trichuris
trichiura, Ascaris lumbricoides, Ancylostoma duodenalis/Necator americanus, Strongyloides
stercoralis, Entorobius vermicularis), life cycles, diagnostic assessment and prevention
- Blood parasites: main parasites (Protozoa: Plasmodium; Trypanosoma sp., Nematodes:
Wuchereria brancofti, Brugia malawi) and life cycles, diagnostic investigation and prevention
- Parasites in other locations: major parasites (Protozoa: Toxoplasma gondii, - Leishmania sp. -
Trichomonas vaginalis, free-living amoebae: Acanthamoeba, Naegleria, Cestodes: Echinococcus
granulosus, Nematodes: Trichinella spiralis, Anisakis sp., Platyhelminthes: Schistomsoma sp.), life
cycles, diagnostic investigation and prevention
- Arthropods: main parasites and vectors; identification and evaluation of epdemiologically relevant
parameters
The main direct and indirect isolation and characterisation techniques (microscopic, molecular
examination, serodiagnosis) will be illustrated for each class
(reference books)
“Lippincott® Illustrated Reviews: Microbiology (Lippincott Illustrated Reviews Series) Cynthia Nau Cornelissen
Ph.D. (Editor), Marcia Metzgar Hobbs PhD (Editor). Series Editor: Harvey RH, Walters Kluwer”
-“Bibliografia e materiale didattico scelti dal docente (presentazioni, articoli scientifici, dispense)”
-“Microbiologia medica, Patrick R. Murray, Ken S. Rosenthal, Michael A. Pfaller, Editore EDRA”
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|
Dates of beginning and end of teaching activities
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From to |
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Delivery mode
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Traditional
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|
Attendance
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not mandatory
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|
Evaluation methods
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Oral exam
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|
|
| Module: Diagnostic laboratory techniques
(objectives)
The main training objective of this teaching is to provide the student with the basic knowledge of
microbiological diagnosis, referring to basic and innovative techniques, aimed at achieving the
training objectives of the professional profile. This enables the student to acquire an overview of
diagnosis by understanding in which biological matrices microorganisms must be sought and whatprecautions must be taken in order to make accurate laboratory diagnoses in the microbiological
field
The main pathogens involved in human pathogenesis will be covered and which laboratory
techniques to adopt depending on the pathogen. Diagnostic algorithms will be illustrated so that
the student will be able to understand the steps of microbiological diagnosis.
By the end of the course, the student will have acquired the main methods and techniques to be
applied for the identification of bacteria, viruses, fungi, parasites and protozoa, with the ability to
apply the most appropriate diagnostic technique.
|
|
Language
|
ITA |
|
Type of certificate
|
Profit certificate
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|
Credits
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2
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Scientific Disciplinary Sector Code
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MED/46
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Contact Hours
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20
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Type of Activity
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Core compulsory activities
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Teacher
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Marcuccilli Fabbio
(syllabus)
Basic concepts in pre-analytical sample in the bacteriology laboratory.
-Sepsis
-Urine culture,
-Liquor and infectious enteritis
-Extraction of nucleic acids. PCR Real-Time. PCR end-point.
-Practical application of manual and automatic methods in the field of molecular virology.
-Description and discussion on the technical validation of the diagnostic reports produced during
the practical sessions.
(reference books)
“Lippincott® Illustrated Reviews: Microbiology (Lippincott Illustrated Reviews Series) Cynthia Nau Cornelissen
Ph.D. (Editor), Marcia Metzgar Hobbs PhD (Editor). Series Editor: Harvey RH, Walters Kluwer”
-“Bibliografia e materiale didattico scelti dal docente (presentazioni, articoli scientifici, dispense)”
-“Microbiologia medica, Patrick R. Murray, Ken S. Rosenthal, Michael A. Pfaller, Editore EDRA”
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Dates of beginning and end of teaching activities
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From to |
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Delivery mode
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Traditional
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Attendance
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not mandatory
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Evaluation methods
|
Oral exam
|
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