The student must learn the general characteristics of antigens, antibodies, cytokines and cells, tissues and organs of the immune system; the mechanisms of regulation of central and peripheral tolerance; the components and molecular mechanisms of innate and adaptive immune responses. Furthermore, the student must learn the causes of human diseases, interpreting the fundamental pathogenetic and pathophysiological mechanisms. The basic principles of the organization of the analysis laboratory are essential goals. Furthermore, the basis for quality in the laboratory will be provided and the UNI EN ISO 9001/15 Certification and Accreditation UNI EN ISO 15189 will be described.
In addition, the teaching aims to provide the theoretical and practical bases of the main techniques for manipulating cell cultures and their applications in the biomedical field. Therefore, the primary objectives will be the learning of the methods of preparation and maintenance of cell cultures, the control of the correct chemical-physical parameters in culture and the cryopreservation procedures of the cells, the main techniques of analysis of cellular response in vitro with particular reference to the immunofluorescence method. Students will also have to acquire knowledge on the main applications of cell cultures in the biomedical field, with specific attention to the field of regenerative medicine.
These objectives will be achieved through lectures and interactive educational activities designed to facilitate learning and improve the ability to deal with and resolve the main ones diagnostic questions from the clinical biochemistry laboratory, the main questions of immunology, physiopathology and of cell culture laboratory protocols employed in clinic and in research.