| General and clinical pathology
(objectives)
The integrated teaching of General and Clinical Pathology aims to provide the student with the fundamentals for knowledge and understanding of the pathogenetic mechanisms of diseases and the analytical procedures used in laboratory diagnosis.
• The General and Cellular Pathology module will provide the student with the main notions of the molecular and cellular mechanisms underlying homeostatic alterations, the response to cell and tissue damage, and the molecular basis of neoplastic transformation and progression.
• The Clinical Pathology and Immunohaematology module will provide the student with the fundamentals of the main laboratory methods applicable to the qualitative and quantitative analysis of pathogenetic determinants and significant biological processes in medicine. Acquisition of the ability to correctly apply the methodologies to detect clinical, functional, and laboratory findings, interpreting them critically also from a physiopathological point of view, for the purposes of diagnosis and prognosis; ability to assess cost/benefit ratios in the choice of diagnostic procedures, taking into account the requirements of both correct clinical methodology and the principles of evidence-based medicine
• The Technical Sciences of Laboratory Medicine module will provide notions of the main techniques of molecular biology, flow cytometry, and cytogenetics for the diagnosis and monitoring of blood diseases and the relevance of laboratory activities for integrated clinical management of the hematological patient.
By the end of the integrated teaching, the student will have acquired the ability to address the main questions of general and cellular pathology and the basic elements in the clinical diagnosis of the main diseases. By applying the experimental method, the student will be able to analyze and correctly interpret experimental data both in the field of health care and in research and to cooperate in clinical reasoning.
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Code
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90331 |
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Language
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ITA |
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Type of certificate
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Profit certificate
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| Module: General and cellular pathology
(objectives)
The integrated teaching of General and Clinical Pathology aims to provide the student with the fundamentals for knowledge and understanding of the pathogenetic mechanisms of diseases and the analytical procedures used in laboratory diagnosis.
• The General and Cellular Pathology module will provide the student with the main notions of the molecular and cellular mechanisms underlying homeostatic alterations, the response to cell and tissue damage, and the molecular basis of neoplastic transformation and progression.
• The Clinical Pathology and Immunohaematology module will provide the student with the fundamentals of the main laboratory methods applicable to the qualitative and quantitative analysis of pathogenetic determinants and significant biological processes in medicine. Acquisition of the ability to correctly apply the methodologies to detect clinical, functional, and laboratory findings, interpreting them critically also from a physiopathological point of view, for the purposes of diagnosis and prognosis; ability to assess cost/benefit ratios in the choice of diagnostic procedures, taking into account the requirements of both correct clinical methodology and the principles of evidence-based medicine
• The Technical Sciences of Laboratory Medicine module will provide notions of the main techniques of molecular biology, flow cytometry, and cytogenetics for the diagnosis and monitoring of blood diseases and the relevance of laboratory activities for integrated clinical management of the hematological patient.
By the end of the integrated teaching, the student will have acquired the ability to address the main questions of general and cellular pathology and the basic elements in the clinical diagnosis of the main diseases. By applying the experimental method, the student will be able to analyze and correctly interpret experimental data both in the field of health care and in research and to cooperate in clinical reasoning.
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Language
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ITA |
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Type of certificate
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Profit certificate
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Credits
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2
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Scientific Disciplinary Sector Code
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MED/04
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Contact Hours
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20
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Type of Activity
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Core compulsory activities
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Teacher
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Capuano Cristina
(syllabus)
• BASIC CONCEPTS OF HEALTH AND DISEASE
• CELLULAR HOMEOSTASIS ALTERATION
o Cellular adaptation: Atrophy, Hypertrophy, Hyperplasia, Metaplasia
o Damage and cell death: Necrosis, Apoptosis
o Cellular Aging
• INFLAMMATION
o Causes and general features of inflammation
o Acute Inflammation: vasculohaematic changes, cellular phase. Inflammatory Exudates. Soluble mediators of inflammation. Outcomes of inflammatory response.
o Chronic Inflammation: morphologic features (acute vs chronic). Impact of macrophages on chronic inflammation. Chronic Interstitial and granulomatous inflammation. Examples of chronic inflammation diseases.
o Systemic effects of inflammation: leukocytosis, acute phase protein, reactive proteic C, erytrosedimentation velocity, fever, septic shock
• MECHANISMS OF TISSUE DAMAGE REPAIR
o Regeneration
o Rpair: Scarring and Fibrosis
o Wound healing: pathologic aspects
• ETHIOPATHOGENETIC CLASSIFICATION OF THE DISEASES
o Extrinsic agents (physical, chemical, biological)
o Intrinsic agents (genetic and malformation diseases)
o Multifactorial diseases
o Hints of Immunopathology
• TUMORS
o Biological and clinical classification criteria
o Biological features: proliferation, invasion, angiogenesis
o Metastatisation
o Carcinogenesis, oncogenes and oncosuppressors, tumour marker concept
(reference books)
• Textbook: Ivan Damjanov MD PhD, Pathology for the Health Professions, 5th Edition, Elsevier; ISBN: 9780323357210; 2016.
• Slides and didactic materials will be provided by the teacher
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Dates of beginning and end of teaching activities
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From to |
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Delivery mode
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Traditional
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Attendance
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Mandatory
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Evaluation methods
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Written test
Oral exam
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| Module: Clinical pathology and immunohematology
(objectives)
The integrated teaching of General and Clinical Pathology aims to provide the student with the fundamentals for knowledge and understanding of the pathogenetic mechanisms of diseases and the analytical procedures used in laboratory diagnosis.
• The General and Cellular Pathology module will provide the student with the main notions of the molecular and cellular mechanisms underlying homeostatic alterations, the response to cell and tissue damage, and the molecular basis of neoplastic transformation and progression.
• The Clinical Pathology and Immunohaematology module will provide the student with the fundamentals of the main laboratory methods applicable to the qualitative and quantitative analysis of pathogenetic determinants and significant biological processes in medicine. Acquisition of the ability to correctly apply the methodologies to detect clinical, functional, and laboratory findings, interpreting them critically also from a physiopathological point of view, for the purposes of diagnosis and prognosis; ability to assess cost/benefit ratios in the choice of diagnostic procedures, taking into account the requirements of both correct clinical methodology and the principles of evidence-based medicine
• The Technical Sciences of Laboratory Medicine module will provide notions of the main techniques of molecular biology, flow cytometry, and cytogenetics for the diagnosis and monitoring of blood diseases and the relevance of laboratory activities for integrated clinical management of the hematological patient.
By the end of the integrated teaching, the student will have acquired the ability to address the main questions of general and cellular pathology and the basic elements in the clinical diagnosis of the main diseases. By applying the experimental method, the student will be able to analyze and correctly interpret experimental data both in the field of health care and in research and to cooperate in clinical reasoning.
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Language
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ITA |
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Type of certificate
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Profit certificate
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Credits
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3
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Scientific Disciplinary Sector Code
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MED/05
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Contact Hours
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30
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Type of Activity
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Core compulsory activities
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Teacher
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Romeo Anna Claudia
(syllabus)
• Definition, limits, and aims of Laboratory Medicine.
• Appropriateness in the choice of laboratory investigations
• Pre-analytical, analytical and post-analytical variability, sensitivity and specificity.
• The report, reference intervals.
• Functional evaluation of organs and tissuesand general pathophysiological states
o THE BLOOD: Clinical biochemistry of haemostasis. Functional evaluation of the biochemi-cal mechanisms underlying haemostasis (vascular phase, coagulation, and fibrinolysis). Qualitative-quantitative clinical biochemistry of blood elements. Evaluation of erythrocyte functionality (haemoglobins, iron metabolism, the study of erythrocyte enzymes, and bio-chemical approach to the study of anaemias). Functional biochemical study of leucocyte populations under normal and pathological conditions. Hints of transfusion medicine
o THE KIDNEY. Pathophysiological evaluation of the kidney and urinary system. Tests for assessing the renal function at the glomerular and tubular level; endocrine functions of the kidney
o THE LIVER: Biochemical evaluation of the biosynthetic (interpretative criteria of the serum protein and individual protein assay) and detoxifying liver functions and structural integrity indexes. Clinical biochemical study of the major functional and structural alterations. Clini-cal biochemical contribution to the differential diagnosis in the course of jaundice.
o THE PANCREAS: Functional evaluation (exocrine and endocrine) and structural integrity of the pancreas
o THE THYROD: Pathophysiological evaluation of the thyroid gland and laboratory diagnosis of thyroid diseases: contribution of evaluation of hypothalamus/pituitary axis
o SYSTEMIC DISEASES: Clinical biochemical approach to the study of certain systemic di-seases: autoimmune and rheumatological diseases, celiac disease, diabetes mellitus, dy-slipidemia
o PREGNANCY: Clinical biochemical evaluation of pregnancy and fetal function; major bio-chemical changes in pregnancy, under normal and pathological conditions; the amniotic fluid biochemistry
(reference books)
Laposata's Laboratory Medicine Diagnosis of Disease in Clinical Laboratory 3rd Edition LANGEC Mc Graw Hill 2018
• Slides and didactic materials will be provided by the teacher
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Dates of beginning and end of teaching activities
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From to |
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Delivery mode
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Traditional
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Attendance
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Mandatory
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|
Evaluation methods
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Written test
Oral exam
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| Module: Technical sciences of laboratory medicine
(objectives)
The integrated teaching of General and Clinical Pathology aims to provide the student with the fundamentals for knowledge and understanding of the pathogenetic mechanisms of diseases and the analytical procedures used in laboratory diagnosis.
• The General and Cellular Pathology module will provide the student with the main notions of the molecular and cellular mechanisms underlying homeostatic alterations, the response to cell and tissue damage, and the molecular basis of neoplastic transformation and progression.
• The Clinical Pathology and Immunohaematology module will provide the student with the fundamentals of the main laboratory methods applicable to the qualitative and quantitative analysis of pathogenetic determinants and significant biological processes in medicine. Acquisition of the ability to correctly apply the methodologies to detect clinical, functional, and laboratory findings, interpreting them critically also from a physiopathological point of view, for the purposes of diagnosis and prognosis; ability to assess cost/benefit ratios in the choice of diagnostic procedures, taking into account the requirements of both correct clinical methodology and the principles of evidence-based medicine
• The Technical Sciences of Laboratory Medicine module will provide notions of the main techniques of molecular biology, flow cytometry, and cytogenetics for the diagnosis and monitoring of blood diseases and the relevance of laboratory activities for integrated clinical management of the hematological patient.
By the end of the integrated teaching, the student will have acquired the ability to address the main questions of general and cellular pathology and the basic elements in the clinical diagnosis of the main diseases. By applying the experimental method, the student will be able to analyze and correctly interpret experimental data both in the field of health care and in research and to cooperate in clinical reasoning.
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Language
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ITA |
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Type of certificate
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Profit certificate
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|
Credits
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2
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Scientific Disciplinary Sector Code
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MED/46
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Contact Hours
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20
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Type of Activity
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Core compulsory activities
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Teacher
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Divona Mariadomenica
(syllabus)
• INTRODUCTION TO INTEGRATED ONCOHEMATOLOGICAL DIAGNOSTICS
o Flow cytometry techniques
o Molecular biology
o Conventional cytogenetics
o Fluorescent in situ hybridization (FISH)
• ISOLATION OF MONONUCLEAR CELLS FROM BONE MARROW ASPIRATE AND PERIPHERAL BLOOD (Ficoll)
• CELL COUNT BY MEANS OF CELL COUNTING CHAMBERS
• NUCLEIC ACID EXTRACTION TECHNIQUES
o Automatic Extractor
o Home Made Techniques
• RT-PCR: BASIC PRINCIPLES AND TECHNICAL ASPECTS
o PCR application in oncohaematology
o Real-time PCR: basic principles and technical aspects
o Application of real-time PCR in the monitoring of minimal resisual disease
• Q-LAMP : BASIC PRINCIPLES AND TECHNICAL ASPECTS
o Application of Q-LAMP in the rapid diagnosis of Acute Promyelocytic Leukemia
• ELECTROPHORETIC TECHNIQUES
o Agarose gel electrophoresis
o Capillary electrophoresis
• CASE REPORT IN THE VALIDATION OF ANALYTICAL DATA.
(reference books)
Slides and didactic materials will be provided by the teacher.
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Dates of beginning and end of teaching activities
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From to |
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Delivery mode
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Traditional
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Attendance
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Mandatory
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Evaluation methods
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Written test
Oral exam
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