Bacteria, viruses and fungi of medical interest.
-Microbiological diagnostics: pathological samples, collection, transport, storage and processing of samples.
-Bacterial infections, direct microscopic examination and culture.
-Viral infections, direct examination and culture.
-Methods of detection of microbial macromolecules. Latex agglutination test. Elisa. Immunofluorescence. Immunohistochemistry.
-Serological diagnosis of infection: methods for documenting the presence and titre of specific antibodies.
-Molecular microbiological diagnostics: extraction of nucleic acids, hybridization methods, amplification of the hybridization signal, post-amplification detection.
-Automation in the clinical microbiology laboratory: automation in serology, identification of bacteria and antibiogram, extraction of nucleic acids and in amplification of nucleic acids.
-Respiratory tract infections: the main clinical manifestations and the laboratory diagnosis of upper and lower respiratory tract infections.
-The most common central nervous system infections (meningitis, encephalitis) and their laboratory diagnosis.
-Infections of the genitourinary system, in relation to the microbes ( bacteria, viruses and fungi) involved and to the various types of laboratory diagnosis. Sexually transmitted diseases (STDs) and urinary tract infections (UTIs).
-Gastrointestinal tract infections: the main infections of the stomach, intestines and liver in relation to their etiologic agents an laboratory diagnosis.
-Skin, bone and joint infections: laboratory diagnostic methods.
-Vascular and cardiac infections, bacteremia, sepsis: main infectious agents and laboratory diagnosis.
-Opportunistic infections and healthcare-related infections (ICA): definitions, risk factors, routes of transmission, prevention and treatment.

“Lippincott® Illustrated Reviews: Microbiology (Lippincott Illustrated Reviews Series) Cynthia Nau Cornelissen Ph.D. (Editor), Marcia Metzgar Hobbs PhD (Editor). Series Editor: Harvey RH, Walters Kluwer”
-“Bibliografia e materiale didattico scelti dal docente (presentazioni, articoli scientifici, dispense)”
-“Microbiologia medica, Patrick R. Murray, Ken S. Rosenthal, Michael A. Pfaller, Editore EDRA”

Introduzione al corso: Concetti di ecologia applicati ai parassiti e zoonosi
-Parassitologia generale
-Protozoi
-Nematodi
-Cestodi
-Trematodi
-Cenni sugli artropodi
-Tecniche di isolamento e caratterizzazione diretta e indiretta dei principali parassiti di interesse umano

“Lippincott® Illustrated Reviews: Microbiology (Lippincott Illustrated Reviews Series) Cynthia Nau Cornelissen Ph.D. (Editor), Marcia Metzgar Hobbs PhD (Editor). Series Editor: Harvey RH, Walters Kluwer”
-“Bibliografia e materiale didattico scelti dal docente (presentazioni, articoli scientifici, dispense)”
-“Microbiologia medica, Patrick R. Murray, Ken S. Rosenthal, Michael A. Pfaller, Editore EDRA”

TECNICHE DIAGNOSTICHE DI VIROLOGIA
-Estrazione degli acidi nucleici.
-PCR Real-time. PCR end-point.
-Applicazione pratica di metodiche manuali ed automatiche in ambito di virologia molecolare.
-Descrizione e discussione sulla validazione tecnica dei referti diagnostici prodotti durante le sessioni pratiche.

TECNICHE DIAGNOSTICHE DI BATTERIOLOGIA
-Concetti di base nella gestione preanalitica del campione nel laboratorio di batteriologia.
-Sepsi
-Urinocoltura
-Liquor
-Enteriti infettive

“Lippincott® Illustrated Reviews: Microbiology (Lippincott Illustrated Reviews Series) Cynthia Nau Cornelissen Ph.D. (Editor), Marcia Metzgar Hobbs PhD (Editor). Series Editor: Harvey RH, Walters Kluwer”
-“Bibliografia e materiale didattico scelti dal docente (presentazioni, articoli scientifici, dispense)”
-“Microbiologia medica, Patrick R. Murray, Ken S. Rosenthal, Michael A. Pfaller, Editore EDRA”