| Molecular biology and clinical pathology
(objectives)
The integrated teaching of Molecular Biology and Clinical Pathology will provide the student with the fundamentals of Molecular Biology and Pathology and will allow the student to translate the basic knowledge acquired into clinical diagnostic applications. The main methodologies used in molecular, biochemical, and cellular diagnostics will be presented, and the conceptual approaches according to which they have been developed will be explained. Different types of tests based on the determination of DNA, RNA, proteins, or metabolites will be introduced and discussed to identify specific pathophysiological states and the main disease markers that measurable in a clinical laboratory will be studied. At the end of the course the student will have acquired the basic elements in the clinical diagnosis of the main pathologies, will be able to use laboratory procedures by applying the experimental method, analyze and correctly interpret experimental data both in the healthcare field and in research and cooperate in clinical reasoning.
|
|
Code
|
90533 |
|
Language
|
ENG |
|
Type of certificate
|
Profit certificate
|
| Module: Molecular biology
(objectives)
The integrated teaching of Molecular Biology and Clinical Pathology will provide the student with the fundamentals of Molecular Biology and Pathology and will allow the student to translate the basic knowledge acquired into clinical diagnostic applications. The main methodologies used in molecular, biochemical, and cellular diagnostics will be presented, and the conceptual approaches according to which they have been developed will be explained.
|
|
Language
|
ENG |
|
Type of certificate
|
Profit certificate
|
|
Credits
|
2
|
|
Scientific Disciplinary Sector Code
|
BIO/11
|
|
Contact Hours
|
20
|
|
Type of Activity
|
Basic compulsory activities
|
|
Teacher
|
Maiani Emiliano
(syllabus)
• Structure and replication of DNA.
• Genome and exome.
• Genome organization: viruses, bacteria, eucaryotic cells.
• Genome alteration and mechanisms of evolution.
• Mechanisms of DNA repair.
• Control of gene expression: promoters and enhancers.
• Structure and function of various RNA species; mrna processing.
• Protein synthesis: translation initiation, elongation, and termination; post-translational modifications.
• Procedures for preparation of nucleic acids and proteins and metabolites from biological samples. Electrophoresis procedures, SDS-PAGE Northern and Western Blot.
(reference books)
● Bruce Alberts et al., Molecular Biology of the Cell”, VII ed., WW Norton & Co.. In alternativa:
● Michael M. Cox, Jennifer Doudna, Michael O'Donnell. Biologia Molecolare. Principi e tecniche, Zanichelli 2013
● Teaching material provided by the teacher during the lessons
|
|
Dates of beginning and end of teaching activities
|
From to |
|
Delivery mode
|
Traditional
|
|
Attendance
|
Mandatory
|
|
Evaluation methods
|
Written test
|
|
|
| Module: Clinical molecular biology
(objectives)
The integrated Course of Molecular Biology and Clinical Pathology will provide the foundations of Molecular Biology and Pathology and will allow the student to translate the basic knowledge acquired into clinical diagnostic applications. The main methodologies used in molecular, biochemical and cellular diagnostics will be presented, and the conceptual approaches according to which they were developed will be explained. Different types of tests based on the determination of DNA, RNA, proteins, or metabolites will be introduced and discussed to identify specific pathophysiological states and the main disease markers that measurable in a clinical laboratory will be studied.
|
|
Language
|
ENG |
|
Type of certificate
|
Profit certificate
|
|
Credits
|
2
|
|
Scientific Disciplinary Sector Code
|
BIO/12
|
|
Contact Hours
|
20
|
|
Type of Activity
|
Core compulsory activities
|
|
Teacher
|
Pieroni Luisa
(syllabus)
● Introduction to clinical molecular biology and molecular biomarkers.
● Clinical Laboratory organization: use of the laboratory, interpretation of results, source of variation, specimen collection.
● Nucleic Acids and proteins analytical techniques and their clinical application (i.e.PCR, Gene sequencing, Hybridization, Microarray ).
● Genome editing and gene therapy concepts,
● Development, and application of CRISPR/Cas9 technique.
● Introduction to omics sciences: genomics, metagenomics, epigenomics, transcriptomics, proteomics and their clinical application.
(reference books)
● Bruce Alberts et al., Molecular Biology of the Cell”, VII ed., WW Norton & Co. In alternativa:
● Michael M. Cox, Jennifer Doudna, Michael O'Donnell. Biologia Molecolare. Principi e tecniche, Zanichelli 2013
● Teaching material provided by the teacher during the lessons
|
|
Dates of beginning and end of teaching activities
|
From to |
|
Delivery mode
|
Traditional
|
|
Attendance
|
Mandatory
|
|
Evaluation methods
|
Written test
|
|
|
| Module: Clinical pathology
(objectives)
At the end of the course the student will have acquired the basic elements in the clinical diagnosis of the main pathologies, will be able to use laboratory procedures by applying the experimental method, analyze and correctly interpret experimental data both in the healthcare field and in research and cooperate in clinical reasoning.
|
|
Language
|
ENG |
|
Type of certificate
|
Profit certificate
|
|
Credits
|
2
|
|
Scientific Disciplinary Sector Code
|
MED/05
|
|
Contact Hours
|
20
|
|
Type of Activity
|
Core compulsory activities
|
|
Teacher
|
Consalvi Silvia
(syllabus)
● Introduction to clinical pathology and evaluation of laboratory tests.
● Complete blood count and related disorders.
● The laboratory in the evaluation of the haemostatic function.
● Outline of transfusion medicine.
● Inflammation biomarkers.
● Principles of the immunological diagnosis of autoimmune diseases.
● Biochemical indicators of liver function and damage, jaundice.
● Parameters of function and renal damage.
● The laboratory in the diagnostic evaluation of diabetes.
● Laboratory diagnosis of dyslipoproteinemias.
(reference books)
● Laposata's Laboratory Medicine Diagnosis of Disease in Clinical Laboratory 3rd Edition – LANGEC – Mc Graw Hill 2018
● Teaching material provided by the teacher during the lessons.
|
|
Dates of beginning and end of teaching activities
|
From to |
|
Delivery mode
|
Traditional
|
|
Attendance
|
Mandatory
|
|
Evaluation methods
|
Written test
|
|
|
|
