Pathological anatomy 2
(objectives)
The Anatomic pathology II course aims to provide the main notions of Cytopathology, Histopathology, Autopsy Technique, Autopsy And Clinical Diagnosis, Digital Pathology and Molecular Pathology Techniques, as well as the basis of laboratory techniques, research and diagnostics in Anatomic Pathology. Mandatory objectives are the knowledge of the basic principles, organization, methodologies and technical laboratory and cognitive-diagnostic activities carried out in the clinical service of Anatomic Pathology. The student must learn the basic principles of Cytopathology, Histopathology, Autopsy Technique, Autopsy and Clinical Diagnosis, Digital Pathology and Molecular Pathology Techniques. They must also acquire knowledge and skills in the main technologies (immunohistochemistry, molecular biology, digitization of histological and cytological preparations) to carry out research and diagnostic activities in Anatomic Pathology. These objectives will be achieved through lectures designed to facilitate learning and improve the ability to solve the main questions of Cytopathology, Histopathology, Autopsy Technique, Autopsy and Clinical Diagnosis, Digital Pathology and Molecular Pathology Techniques and laboratory protocols used in research and in the diagnostic.
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Code
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90340 |
Language
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ITA |
Type of certificate
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Profit certificate
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Module: Elements of autopsy, cytopathological, histological diagnostics, autopsy techniques
(objectives)
The student will be able (i) to know the basic macroscopic and optical microscopic anatomical lesions of the main pathologies of the various apparatus and (ii) to understand the role of histological, immunohistochemical and molecular biology investigations in the anatomopathological diagnostic process both in the care diagnostic workflow and in the realization and drafting of basic and applied research projects. Essential objectives • the acquisition of basic knowledge of the histopathological characteristics of the main pathologies of the various apparatuses (hemolympopoietic, lungs, breast, thyroid, oesophagus, stomach, intestine, liver, kidneys, bladder, prostate, testicles, cervix, uterus and ovary). • the acquisition of basic knowledge for performing immunohistochemical reactions • the acquisition of basic notions for the use of the main antibodies for immunohistochemical reactions • the acquisition of basic notions for the use of the main detection systems and chromogens in immunohistochemistry • the acquisition of basic knowledge about the use of the main antibodies used in the pathological diagnostics
The acquisition of the following basic knowledge is an essential objective: • to evaluate the correct preparation of a cytological samples; • the criteria used in analyzing cytological samples under the microscope; • the morphological characteristics of normal, reactive, atypical, dysplastic and neoplastic cells; • to recognize under the microscope normal and pathological human cells, both in diagnostic and care processes and in the execution and drafting of basic and applied research projects.
Essential objectives • The acquisition of knowledge of the autopsy technique • Acquisition of basic knowledge of the main macroscopic alterations of organs detectable at autopsy examination
The module aims to provide the basic knowledge of "AUTOPSY AND CLINICAL DIAGNOSIS " and " ELEMENTS OF DIGITAL PATHOLOGY" to the students. In "AUTOPSY AND CLINICAL DIAGNOSIS " are essential objectives: the acquisition of the knowledge of the definition, history and purpose of the autopsy, the knowledge of the autopsy as a tool for clinical control, the knowledge of the autopsy as a risk management tool, the knowledge of the autopsy as a tool for improving the quality of clinical-assistance pathways, the knowledge of the safety procedures to be adopted during autopsy investigation, the knowledge of the general rules of biosafety in the autopsy room, the knowledge of the value of the autopsy report, the knowledge of post-mortem phenomena. In " ELEMENTS OF DIGITAL PATHOLOGY" are essential objectives: the acquisition of the knowledge of the "Digital Slide" and the Technologies for the digitalization of histological slides; the acquisition of the knowledge of the role of Digital Pathology; the acquisition of the knowledge of Telepathology; the acquisition of the knowledge of privacy and data management.
These objectives will be achieved through lectures, exercises and interactive teaching activities.
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Language
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ITA |
Type of certificate
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Profit certificate
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Credits
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2
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Scientific Disciplinary Sector Code
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MED/08
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Contact Hours
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20
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Personal Study Hours
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-
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Type of Activity
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Core compulsory activities
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Credits
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2
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Scientific Disciplinary Sector Code
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MED/08
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Contact Hours
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20
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Personal Study Hours
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-
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Type of Activity
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Core compulsory activities
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Teacher
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Martini Maurizio
(syllabus)
BASIC HISTOLOGICAL AND CYTOLOGICAL TECHNIQUES: o Main techniques of fixation and preparation of histological samples o Main techniques of fixation and preparation of biological fluids o Techniques of paraffin inclusion of histological samples o Embedding and paraffin cutting techniques o Preparation techniques for fresh samples o Artifacts from technical set-up o Principles of operation and use of the instruments o Safety in the Pathological Anatomy laboratory ANCILLARY HISTOLOGICAL AND CYTOLOGICAL TECHNIQUES: o Histochemical staining techniques o Immunohistochemical investigation techniques o Immunofluorescence techniques (direct and indirect) o Outlines of electron microscopy techniques AUTOPTIC HISTOLOGICAL AND CYTOLOGICAL TECHNIQUES: o Main techniques in the execution of the diagnostic check o Main techniques of organ harvesting and sampling in the autopsy diagnosis MOBILE RESPONSE TO DAMAGE: o Cellular response to ischemia (definition of ischemia and hypoxia, types of hypoxia; hypoxic, anemic, stagnant, ischemic, histotoxic) o Hot ischemia and cold ischemia o Times of ischemia and cell susceptibility o Ischemic cell damage, dropsy degeneration, vacuolar degeneration, cloudy swelling o Reversible and irreversible subcellular changes associated with hydropic swelling o Microscopic aspects of cell death, Concept of oncosis, Coagulative necrosis, necrosis colliquative and apoptosis PRINCIPLES OF OPTICS: o Principles of optics
Converging and diverging lenses o Chromatic Aberrations o Image formation MICROSCOPY: o Principles of optical microscopy o Brightfield microscope o Darkfield microscopy ELECTRON MICROSCOPY: o Principles of electron microscopy o Ultrastructural microscopy applications
(reference books)
Title: Pathological Anatomy The basics Author Aldo Scarpa, Luigi Ruco Publisher Edra year 2007
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
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Attendance
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Mandatory
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Evaluation methods
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Written test
Oral exam
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Teacher
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Larocca Luigi Maria
(syllabus)
o Main techniques of fixation and preparation of histological samples o Main techniques of fixation and preparation of biological fluids o Techniques of paraffin inclusion of histological samples o Embedding and paraffin cutting techniques o Preparation techniques for fresh samples o Artifacts from technical set-up o Principles of operation and use of the instruments o Safety in the Pathological Anatomy laboratory ANCILLARY HISTOLOGICAL AND CYTOLOGICAL TECHNIQUES: o Histochemical staining techniques o Immunohistochemical investigation techniques o Immunofluorescence techniques (direct and indirect) o Outlines of electron microscopy techniques AUTOPTIC HISTOLOGICAL AND CYTOLOGICAL TECHNIQUES: o Main techniques in the execution of the diagnostic check o Main techniques of organ harvesting and sampling in the autopsy diagnosis MOBILE RESPONSE TO DAMAGE: o Cellular response to ischemia (definition of ischemia and hypoxia, types of hypoxia; hypoxic, anemic, stagnant, ischemic, histotoxic) o Hot ischemia and cold ischemia o Times of ischemia and cell susceptibility o Ischemic cell damage, dropsy degeneration, vacuolar degeneration, cloudy swelling o Reversible and irreversible subcellular changes associated with hydropic swelling o Microscopic aspects of cell death, Concept of oncosis, Coagulative necrosis, necrosis colliquative and apoptosis PRINCIPLES OF OPTICS: o Principles of optics
Converging and diverging lenses o Chromatic Aberrations o Image formation MICROSCOPY: o Principles of optical microscopy o Brightfield microscope o Darkfield microscopy o Fluorescence microscope ELECTRON MICROSCOPY: o Principles of electron microscopy o Ultrastructural microscopy applications
(reference books)
Pathological Anatomy The basics Author Aldo Scarpa, Luigi Ruco Publisher Edra year 2007
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
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Attendance
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Mandatory
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Evaluation methods
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Written test
Oral exam
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Module: Molecular pathology techniques
(objectives)
The essential objectives of the course are: the acquisition of basic knowledge about the main techniques used in the molecular pathology laboratory for the extraction of nucleic acids such as PCR and F.I.S.H. These objectives will be achieved through lectures and interactive teaching activities, designed to facilitate learning and improve the ability to solve problems related to the execution of molecular pathology techniques.
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Language
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ITA |
Type of certificate
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Profit certificate
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Credits
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1
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Scientific Disciplinary Sector Code
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MED/46
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Contact Hours
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10
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Type of Activity
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Core compulsory activities
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Teacher
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D'Angelo Martina
(syllabus)
Main methods of nucleic acid extraction: from the pre-analytical phase to the evaluation of the extract PCR preparation techniques and applications in molecular pathology F.I.S.H. method: process and sector applications
(reference books)
Dr. D'Angelo will provide the student with educational material, such as handouts, presentations and scientific articles
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
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Attendance
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Mandatory
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Evaluation methods
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Written test
Oral exam
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