Molecular biology and clinical pathology
(objectives)
To acquire learn basic knowledge on fundamental processes of molecular biology and their regulation, necessary to understand: • The pathogenic mechanisms of diseases • Molecular mechanisms involved in the therapeutic intervention • Biotechnology applications of medical relevance, including the principal methods for the study of nucleic acids and of their application for diagnostic and research purpose • Clinical laboratory organization, clinical biomarker significance, sample variability • Tests allowing determination of DNA, RNA, proteins and metabolites diagnostic and prognostic biomarkers of specific disease currently use in clinical practice • Basic elements of clinical laboratory procedures application, data analysis and interpretation both in research and clinical practice.
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Code
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90533 |
Language
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ENG |
Type of certificate
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Profit certificate
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Module: Molecular biology
(objectives)
To acquire learn basic knowledge on fundamental processes of molecular biology and their regulation, necessary to understand all tests allowing determination of DNA, RNA, proteins and metabolites diagnostic and prognostic biomarkers of specific disease currently use in clinical practice.
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Language
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ENG |
Type of certificate
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Profit certificate
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Credits
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2
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Scientific Disciplinary Sector Code
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BIO/11
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Contact Hours
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20
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Type of Activity
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Basic compulsory activities
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Teacher
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Montagna Costanza
(syllabus)
- Structure and replication of DNA; genome and exome; genome organization: viruses, bacteria, eucaryotic cells; genome alteration and mechanisms of evolution; mechanisms of DNA repair; control of gene expression: promoters and enhancers. - Structure and function of various RNA species; mRNA processing. - Genome editing and gene therapy concepts, development, and application of CRISPR/Cas9 technique. Fundamentals of protein synthesis: translation initiation, elongation, and termination; post-translational modifications.
(reference books)
- WATSON James D , BAKER Tania A , BELL Stephen P , GANN Alexander , LEVINE Michael , LOSICK Richard Molecular Biology of the Gene (7th ed) COLD SPRING HARBOR LABORATORY PRESS - Michael M. Cox, Jennifer Doudna, Michael O'Donnell. Molecular Biology: Principles and Practice; W H Freeman & Co; 2 edition (16 March 2015)
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
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Attendance
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Mandatory
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Evaluation methods
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Written test
Oral exam
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Module: Clinical molecular biology
(objectives)
The integrated Course of Molecular Biology and Clinical Pathology will provide the foundations of Molecular Biology and Pathology and will allow the student to translate the basic knowledge acquired into clinical diagnostic applications. The main methodologies used in molecular, biochemical and cellular diagnostics will be presented, and the conceptual approaches according to which they were developed will be explained.
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Language
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ENG |
Type of certificate
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Profit certificate
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Credits
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2
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Scientific Disciplinary Sector Code
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BIO/12
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Contact Hours
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20
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Type of Activity
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Core compulsory activities
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Teacher
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Pieroni Luisa
(syllabus)
• Introduction to clinical molecular biology and molecular biomarkers. Laboratory organization. Sample variability: biological, preanalytical, analyitical, and postanalytical. Diagnostic sensitivity and sensibility; • Structure and function DNA, RNA, proteins and metabolites; • Procedures for preparation of nucleic acids and proteins and metabolites from biological samples; • PCR, Gene sequencing (NGS, Exome Sequencing, RFLP); • Introduction to omics sciences: trascrittomics, proteomics and metabolomics and their clinical application. Microarray. Proteome analysis of different matrix (saliva, plasma, serum), LC-MS and metabolites.
(reference books)
• Michael M. Cox, Jennifer Doudna, Michael O'Donnell. Molecular Biology: Principles and Practice; W H Freeman & Co; 2 edition (16 March 2015) • Teaching material provided by the teacher during the lessons
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
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Attendance
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Mandatory
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Evaluation methods
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Written test
Oral exam
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Module: Clinical pathology
(objectives)
The integrated course of Molecular Biology and Clinical Pathology will provide the student with the foundations of Molecular Biology and Pathology and will allow the student to translate the basic knowledge acquired into clinical diagnostic applications. At the end of the course, the student will have acquired the basic elements in clinical diagnostics of the main pathologies, will be able to use laboratory procedures by applying the experimental method, correctly analyze and interpret experimental data both in the field of care and in research and cooperate in clinical reasoning.
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Language
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ENG |
Type of certificate
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Profit certificate
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Credits
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2
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Scientific Disciplinary Sector Code
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MED/05
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Contact Hours
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20
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Type of Activity
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Core compulsory activities
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Teacher
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Romeo Anna Claudia
(syllabus)
• Introduction to clinical pathology and evaluation of laboratory tests • Complete blood count and related disorders • The laboratory in the evaluation of the haemostatic function • Outline of transfusion medicine • Biochemical indicators of liver function and damage, jaundice • Parameters of function and renal damage • The laboratory in the diagnostic evaluation of diabetes • Laboratory diagnosis of dyslipoproteinemias • Electrolytes, blood gases, calcium, magnesium, phosphorus • Determination of plasma proteins and acute phase markers • Principles of the immunological diagnosis of autoimmune diseases.
(reference books)
• Laposata's Laboratory Medicine Diagnosis of Disease in Clinical Laboratory 3rd Edition – LANGEC – Mc Graw Hill 2018 • Teaching material provided by the teacher during the lessons
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Dates of beginning and end of teaching activities
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From to |
Delivery mode
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Traditional
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Attendance
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Mandatory
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Evaluation methods
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Written test
Oral exam
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